Description
| Product Name: | Human APOD Recombinant Protein |
| Product Code: | RPPB5719 |
| Size: | 10µg |
| Species: | Human |
| Target: | APOD |
| Synonyms: | Apolipoprotein D, Apo-D, ApoD. |
| Source: | HEK293 Cells |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4�m) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility: | It is recommended to add 200�l deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | QAFHLGKCPN PPVQENFDVN KYLGRWYEIE KIPTTFENGR CIQANYSLME NGKIKVLNQE LRADGTVNQI EGEATPVNLT EPAKLEVKFS WFMPSAPYWI LATDYENYAL VYSCTCIIQL FHVDFAWILA RNPNLPPETV DSLKNILTSN NIDVKKMTVT DQVNCPKLSH HHHHH |
Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid. Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer's disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.
Apolipoprotein-D Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (aa 21-189) containing a total of 175 amino acids, having a molecular mass of 20.1kDa (calculated) and fused to a 6 aa His tag at C-Terminus.The Human APOD is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | APOD: APOD occurs in the macromolecular complex with lecithin- cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Belongs to the calycin superfamily. Lipocalin family. |
| UniProt Protein Details: | Protein type:Lipid-binding; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 3q29 Cellular Component: cell soma; dendrite; endoplasmic reticulum; extracellular space; perinuclear region of cytoplasm Molecular Function:cholesterol binding; protein binding Biological Process: axon regeneration in the peripheral nervous system; brain development; glucose metabolic process; lipid metabolic process; negative regulation of focal adhesion formation; negative regulation of protein import into nucleus; negative regulation of smooth muscle cell proliferation; response to axon injury; response to drug; response to reactive oxygen species; tissue regeneration |
| NCBI Summary: | This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008] |
| UniProt Code: | P05090 |
| NCBI GenInfo Identifier: | 114034 |
| NCBI Gene ID: | 347 |
| NCBI Accession: | P05090.1 |
| UniProt Secondary Accession: | P05090,Q6IBG6, B2R579, D3DNW6, |
| UniProt Related Accession: | P05090 |
| Molecular Weight: | 21,276 Da |
| NCBI Full Name: | Apolipoprotein D |
| NCBI Synonym Full Names: | apolipoprotein D |
| NCBI Official Symbol: | APOD�� |
| NCBI Protein Information: | apolipoprotein D |
| UniProt Protein Name: | Apolipoprotein D |
| Protein Family: | Apolipoprotein |
| UniProt Gene Name: | APOD�� |
| UniProt Entry Name: | APOD_HUMAN |
