Description
| Product Name: | Human AHSP Recombinant Protein |
| Product Code: | RPPB2721 |
| Size: | 50µg |
| Species: | Human |
| Target: | AHSP |
| Synonyms: | Alpha-hemoglobin-stabilizing protein, Erythroid-associated factor, Erythroid differentiation-related factor, AHSP, EDRF, ERAF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The AHSP protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS |
Alpha-hemoglobin stabilizing protein (AHSP) is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of A-hemoglobin during normal erythroid cell development. AHSP specifically protects free A-hemoglobin from precipitation in live cells and in solution. AHSP is expected to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Furthermore, AHSP promotes alpha globin chain stability in human erythropoiesis. In addition, the AHSP stabilizes the alpha-Hb chain, thus avoiding its precipitation and its ability to generate ROS, which is implicated in cell death. AHSP is expressed in blood and bone marrow. AHSP subunit is a monomer, it forms a heterodimer with free alpha-hemoglobin. On the other hand, AHSP does not bind beta-hemoglobin nor alpha2beta2 hemoglobin A. AHSP is downregulated in TSEs (transmissible spongiform encephalopathies).
AHSP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 102 amino acids (1-102 a.a.) and having a molecular mass of 11.8kDa.The AHSP is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AHSP: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Belongs to the AHSP family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: hemoglobin complex Molecular Function:hemoglobin binding; unfolded protein binding Biological Process: protein folding; protein stabilization; hemoglobin metabolic process; hemopoiesis |
| UniProt Code: | Q9NZD4 |
| NCBI GenInfo Identifier: | 23813669 |
| NCBI Gene ID: | 51327 |
| NCBI Accession: | Q9NZD4.1 |
| UniProt Secondary Accession: | Q9NZD4,Q8TD01, |
| UniProt Related Accession: | Q9NZD4 |
| Molecular Weight: | 102 |
| NCBI Full Name: | Alpha-hemoglobin-stabilizing protein |
| NCBI Synonym Full Names: | alpha hemoglobin stabilizing protein |
| NCBI Official Symbol: | AHSP�� |
| NCBI Official Synonym Symbols: | EDRF; ERAF�� |
| NCBI Protein Information: | alpha-hemoglobin-stabilizing protein; erythroid associated factor; erythroid-associated factor; alpha hemoglobin stabilising protein; erythroid differentiation-related factor; erythroid differentiation associated factor |
| UniProt Protein Name: | Alpha-hemoglobin-stabilizing protein |
| UniProt Synonym Protein Names: | Erythroid differentiation-related factor; Erythroid-associated factor |
| Protein Family: | Alpha-hemoglobin-stabilizing protein |
| UniProt Gene Name: | AHSP�� |
| UniProt Entry Name: | AHSP_HUMAN |
