Description
| Product Name: | Nanodisc Human AGRA2 Protein |
| SKU: | HDFP182 |
| Size: | 10μg |
| Target: | AGRA2 |
| Tag: | C-Flag Tag |
| Expression Host: | HEK293 |
| Conjugate: | Unconjugated |
| Synonyms: | GPR124, TEM5 |
| Protein Families: | Transmembrane, Druggable Genome. |
| Protein Description: | Human AGRA2 full length protein-synthetic nanodisc |
| Protein Pathways: | Angiogenesis. |
| Molecular Weight: | The human full length AGRA2 protein has a MW of 142.6kDa |
Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed:30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).[UniProtKB/Swiss-Prot Function]
| Uniprot ID: | Q96PE1 |
| Formulation & Reconstitution: | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping: | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Usage: | Research use only |
