Description
| Product Name: | Human Adipsin Recombinant Protein |
| Product Code: | RPPB2704 |
| Size: | 20µg |
| Species: | Human |
| Target: | Adipsin |
| Synonyms: | Complement factor D, EC 3.4.21.46, Adipsin, C3 convertase activator, Properdin factor D, CFD, DF, PFD, ADN. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | Adipsin protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MILGGREAEA HARPYMASVQ LNGAHLCGGV LVAEQWVLSA AHCLEDAADG KVQVLLGAHS LSQPEPSKRL YDVLRAVPHP DSQPDTIDHD LLLLQLSEKA TLGPAVRPLP WQRVDRDVAP GTLCDVAGWG IVNHAGRRPD SLQHVLLPVL DRATCNRRTH HDGAITERLM CAESNRRDSC KGDSGGPLVC GGVLEGVVTS GSRVCGNRKK PGIYTRVASY AAWIDSVLA |
Complement Factor D (Adipsin), which belongs to the trypsin family of peptidases, is involved in the alternative complement pathway of the complement system where it cleaves factor B. In the alternative complement pathway, Adipsin is best known for its role in humoral suppression of infectious agents. In addition, Adipsin is a serine protease which is secreted by adipocytes into the bloodstream. Ultimately, Adipsin has a high level of expression in fat, proposing a role for adipose tissue in immune system biology.
Adipsin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 249 amino acids (26-253 a.a) and having a molecular mass of 26.6kDa.Adipsin is fused to a 21 amino acid His-tag at N-terminus.
| UniProt Protein Function: | CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family. |
| UniProt Protein Details: | Protein type:Secreted; EC 3.4.21.46; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: extracellular region Molecular Function:serine-type endopeptidase activity; serine-type peptidase activity Biological Process: complement activation; complement activation, alternative pathway; platelet degranulation Disease: Complement Factor D Deficiency |
| NCBI Summary: | This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P00746 |
| NCBI GenInfo Identifier: | 158515408 |
| NCBI Gene ID: | 1675 |
| NCBI Accession: | P00746.5 |
| UniProt Secondary Accession: | P00746,Q5U5S1, Q86VJ5, Q8N4E0, Q8WZB4, B4DV76, |
| UniProt Related Accession: | P00746 |
| Molecular Weight: | 27,033 Da |
| NCBI Full Name: | Complement factor D |
| NCBI Synonym Full Names: | complement factor D |
| NCBI Official Symbol: | CFD�� |
| NCBI Official Synonym Symbols: | DF; ADN; PFD; ADIPSIN�� |
| NCBI Protein Information: | complement factor D |
| UniProt Protein Name: | Complement factor D |
| UniProt Synonym Protein Names: | Adipsin; C3 convertase activator; Properdin factor D |
| Protein Family: | Complement factor |
| UniProt Gene Name: | CFD�� |
| UniProt Entry Name: | CFAD_HUMAN |
