Description
| Product Name: | Human ACAT2 Recombinant Protein |
| Product Code: | RPPB5772 |
| Size: | 20µg |
| Species: | Human |
| Target: | ACAT2 |
| Synonyms: | Acetyl-CoA acetyltransferase cytosolic, Cytosolic acetoacetyl-CoA thiolase, ACAT2, Acetyl CoA transferase-like protein, ACAT-2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | ACAT2 Human solution containing 20mM Tris pH-8, 0.1M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QR |
ACAT2 enzyme participates in lipid metabolism. ACAT2 takes part in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus.
ACAT2 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 433 amino acids (1-397 a.a.) and having a molecular mass of 45.4 kDa. The ACAT2 is fused to 36 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ACAT2: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - butanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Acetyltransferase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 6q25.3 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:acetyl-CoA C-acetyltransferase activity Biological Process: lipid metabolic process Disease: Acetyl-coa Acetyltransferase-2 Deficiency |
| NCBI Summary: | The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q9BWD1 |
| NCBI GenInfo Identifier: | 52000838 |
| NCBI Gene ID: | 39 |
| NCBI Accession: | Q9BWD1.2 |
| UniProt Related Accession: | Q9BWD1 |
| Molecular Weight: | 41 kDa |
| NCBI Full Name: | Acetyl-CoA acetyltransferase, cytosolic |
| NCBI Synonym Full Names: | acetyl-CoA acetyltransferase 2 |
| NCBI Official Symbol: | ACAT2�� |
| NCBI Protein Information: | acetyl-CoA acetyltransferase, cytosolic |
| UniProt Protein Name: | Acetyl-CoA acetyltransferase, cytosolic |
| UniProt Synonym Protein Names: | Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase |
| Protein Family: | Acetyl-CoA acetyltransferase |
| UniProt Gene Name: | ACAT2�� |
| UniProt Entry Name: | THIC_HUMAN |
