Description
| Product Name: | Nanodisc Human β2AR-GPR75-BRIL Protein |
| SKU: | HDFP1470 |
| Size: | 10μg |
| Target: | GPR75 |
| Tag: | C-Flag Tag |
| Expression Host: | HEK293 |
| Conjugate: | Unconjugated |
| Synonyms: | GPRchr2; WI31133 |
| Protein Families: | Druggable Genome, GPCR, Transmembrane. |
| Protein Description: | Human β2AR-GPR75-BRIL full length protein-synthetic nanodisc |
| Molecular Weight: | The human full length β2AR-GPR75-BRIL protein has a MW of 59.4 kDa |
GPR75 is a member of the G protein-coupled receptor family and is a novel target for the clinical treatment of obesity. Human GPR75 haploinsufficiency exhibits a striking phenotype of low body fat, and GPR75 knockout mice are hypophagic and thin, improving glucose tolerance and insulin sensitivity. To enhance the expression level of GPR75, twenty-four amino acids from the β2AR receptor (MGQPGNGSAFLLAPNRSHAPDHDV) were used to replace the original residues1-31 in a modified version of the GPR75 receptor. Furthermore, the BRIL sequence was used to replace the original ICL3 loop (237-306) to enhance receptor stability. BRIL is thermostabilized apocytochrome b562 with mutations M7W, H102I, R106L, PDB ID 1M6T.
| Uniprot ID: | O95800 |
| Formulation & Reconstitution: | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for |
| Storage & Shipping: | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Usage: | Research use only |
