Description
| 抗体名: | HSD17B1 Antibody (PACO55550) |
| 抗体コード: | PACO55550 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Estradiol 17-beta-dehydrogenase 1 protein (268-328AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO55550 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO55550 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO55550 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. |
| シノニム: | Estradiol 17-beta-dehydrogenase 1 (EC 1.1.1.62) (17-beta-hydroxysteroid dehydrogenase type 1) (17-beta-HSD 1) (20 alpha-hydroxysteroid dehydrogenase) (20-alpha-HSD) (E2DH) (Placental 17-beta-hydroxysteroid dehydrogenase) (Short chain dehydrogenase/reductase family 28C member 1), HSD17B1, E17KSR EDH17B1 EDH17B2 EDHB17 SDR28C1 |
| UniProt Protein Function: | HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Oxidoreductase Chromosomal Location of Human Ortholog: 17q11-q21 Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol Molecular Function:estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity Biological Process: steroid metabolic process; estrogen metabolic process; steroid biosynthetic process; estrogen biosynthetic process |
| UniProt Code: | P14061 |
| NCBI GenInfo Identifier: | 313104233 |
| NCBI Gene ID: | 3292 |
| NCBI Accession: | P14061.3 |
| UniProt Secondary Accession: | P14061,Q2M2L8, B3KXS1, |
| UniProt Related Accession: | P14061 |
| Molecular Weight: | 34,950 Da |
| NCBI Full Name: | Estradiol 17-beta-dehydrogenase 1 |
| NCBI Synonym Full Names: | hydroxysteroid (17-beta) dehydrogenase 1 |
| NCBI Official Symbol: | HSD17B1 |
| NCBI Official Synonym Symbols: | HSD17; EDHB17; EDH17B2; SDR28C1 |
| NCBI Protein Information: | estradiol 17-beta-dehydrogenase 1; E2DH; 20-alpha-HSD; 17-beta-HSD 1; estradiol 17-beta-dehydrogenase-1; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 1; placental 17-beta-hydroxysteroid dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 1 isoform; short chain dehydrogenase/reductase family 28CE, member 1 |
| UniProt Protein Name: | Estradiol 17-beta-dehydrogenase 1 |
| UniProt Synonym Protein Names: | 17-beta-hydroxysteroid dehydrogenase type 1; 17-beta-HSD 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Placental 17-beta-hydroxysteroid dehydrogenase |
| Protein Family: | Estradiol 17-beta-dehydrogenase |
| UniProt Gene Name: | HSD17B1 |
| UniProt Entry Name: | DHB1_HUMAN |
| Antibodies |
| Anti-HSD17B1 Antibody (CAB10839) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
