Description
| 抗体名: | HMGCS1 Antibody (PACO60721) |
| 抗体コード: | PACO60721 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic protein (360-520AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60721 diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO60721 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60721 diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. |
| シノニム: | Hydroxymethylglutaryl-CoA synthase, cytoplasmic (HMG-CoA synthase) (EC 2.3.3.10) (3-hydroxy-3-methylglutaryl coenzyme A synthase), HMGCS1, HMGCS |
| UniProt Code: | Q01581 |
| NCBI GenInfo Identifier: | 1708239 |
| NCBI Gene ID: | 3157 |
| NCBI Accession: | Q01581.2 |
| UniProt Related Accession: | Q01581 |
| Molecular Weight: | |
| NCBI Full Name: | Hydroxymethylglutaryl-CoA synthase, cytoplasmic |
| NCBI Synonym Full Names: | 3-hydroxy-3-methylglutaryl-CoA synthase 1 |
| NCBI Official Symbol: | HMGCS1 |
| NCBI Official Synonym Symbols: | HMGCS |
| NCBI Protein Information: | hydroxymethylglutaryl-CoA synthase, cytoplasmic |
| UniProt Protein Name: | Hydroxymethylglutaryl-CoA synthase, cytoplasmic |
| UniProt Synonym Protein Names: | 3-hydroxy-3-methylglutaryl coenzyme A synthase |
| UniProt Gene Name: | HMGCS1 |
| UniProt Entry Name: | HMCS1_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
