The Harmonin Polyclonal Antibody (CAB4368) is a valuable tool for researchers studying the protein harmonin, a key component in the auditory system essential for hearing. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for various research applications.Harmonin is known for its role in maintaining the structure and function of hair cells in the inner ear, crucial for auditory transduction. By targeting harmonin with this antibody, researchers can detect and analyze the protein in different cell types, providing insights into its function and potential implications for hearing disorders.
Research into harmonin is important for understanding the molecular mechanisms underlying hearing loss and related conditions, offering potential targets for therapeutic interventions. The Harmonin Polyclonal Antibody is validated for use in techniques such as Western blot, immunofluorescence, and immunohistochemistry, providing reliable results for studies in auditory biology and sensory neuroscience.
Product Name:
USH1C Rabbit Polyclonal Antibody
SKU:
CAB4368
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of zebrafish USH1C (NP_001035018.1).
HT-29,Mouse kidney,Mouse small intestine,Rat testis,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, cytoskeleton, cytosol.
Calculated MW:
45kDa/58kDa/60kDa/62kDa/101kDa
Observed MW:
73kDa
Predicted to enable actin filament binding activity. Acts upstream of or within neuron development; startle response; and synapse organization. Predicted to be part of stereocilia ankle link complex. Predicted to be active in several cellular components, including cilium; photoreceptor inner segment; and stereocilium tip. Is expressed in brain; hair cell; pleuroperitoneal region; sensory system; and trunk musculature. Used to study Usher syndrome. Human ortholog(s) of this gene implicated in Usher syndrome; Usher syndrome type 1; Usher syndrome type 1C; and autosomal recessive nonsyndromic deafness 18A. Orthologous to human USH1C (USH1 protein network component harmonin).
Purification Method:
Affinity purification
Gene ID:
564412
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using USH1C antibody (CAB4368) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
