Description
| 抗体名: | GUCA2B Antibody (PACO35338) |
| 抗体コード: | PACO35338 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Guanylate cyclase activator 2B protein (37-112AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO35338 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO35338 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO35338 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. |
| シノニム: | Guanylate cyclase activator 2B [Cleaved into: Guanylate cyclase C-activating peptide 2 (Guanylate cyclase C-activating peptide II) (GCAP-II); Uroguanylin (UGN)], GUCA2B |
| UniProt Protein Function: | GUCA2B: Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. Belongs to the guanylin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p34-p33 Molecular Function:calcium sensitive guanylate cyclase activator activity |
| NCBI Summary: | This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products, including uroguanylin, a member of the guanylin family of peptides and an endogenous ligand of the guanylate cyclase-C receptor. Binding of this peptide to its cognate receptor stimulates an increase in cyclic GMP and may regulate salt and water homeostasis in the intestine and kidneys. [provided by RefSeq, Nov 2015] |
| UniProt Code: | Q16661 |
| NCBI GenInfo Identifier: | 2495130 |
| NCBI Gene ID: | 2981 |
| NCBI Accession: | Q16661.1 |
| UniProt Secondary Accession: | Q16661,Q52LV0, |
| UniProt Related Accession: | Q16661 |
| Molecular Weight: | 12,069 Da |
| NCBI Full Name: | Guanylate cyclase activator 2B |
| NCBI Synonym Full Names: | guanylate cyclase activator 2B |
| NCBI Official Symbol: | GUCA2B |
| NCBI Official Synonym Symbols: | UGN; GCAP-II |
| NCBI Protein Information: | guanylate cyclase activator 2B |
| UniProt Protein Name: | Guanylate cyclase activator 2B |
| UniProt Synonym Protein Names: | Guanylate cyclase C-activating peptide 2Alternative name(s):Guanylate cyclase C-activating peptide II; GCAP-II |
| Protein Family: | Guanylate cyclase activator |
| UniProt Gene Name: | GUCA2B |
| UniProt Entry Name: | GUC2B_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
