Description
| 抗体名: | GPHN Antibody (PACO58777) |
| 抗体コード: | PACO58777 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Gephyrin protein (360-460AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58777 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO58777 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. |
| シノニム: | Gephyrin [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (EC 2.7.7.75) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (EC 2.10.1.1) (Domain E)], GPHN, GPH KIAA1385 |
| UniProt Protein Function: | gephyrin: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. Homotrimer. Interacts with GABARAP. Inhibited by copper and tungsten. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.7.75; Adaptor/scaffold; EC 2.10.1.1 Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: cytoplasm; dendrite; plasma membrane; postsynaptic membrane Molecular Function:protein binding Biological Process: Mo-molybdopterin cofactor biosynthetic process; molybdenum incorporation into molybdenum-molybdopterin complex; molybdopterin cofactor biosynthetic process Disease: Hyperekplexia, Hereditary 1; Molybdenum Cofactor Deficiency, Complementation Group C |
| NCBI Summary: | This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NQX3 |
| NCBI GenInfo Identifier: | 13431554 |
| NCBI Gene ID: | 10243 |
| NCBI Accession: | Q9NQX3.1 |
| UniProt Secondary Accession: | Q9NQX3,Q96KU4, Q9H4E9, Q9P2G2, |
| UniProt Related Accession: | Q9NQX3 |
| Molecular Weight: | 83,448 Da |
| NCBI Full Name: | Gephyrin |
| NCBI Synonym Full Names: | gephyrin |
| NCBI Official Symbol: | GPHN |
| NCBI Official Synonym Symbols: | GPH; GEPH; HKPX1; GPHRYN; MOCODC |
| NCBI Protein Information: | gephyrin |
| UniProt Protein Name: | Gephyrin |
| UniProt Synonym Protein Names: | Domain E |
| Protein Family: | Gephyrin |
| UniProt Gene Name: | GPHN |
| UniProt Entry Name: | GEPH_HUMAN |
