Description
| 抗体名: | GPA33 Antibody (PACO40202) |
| 抗体コード: | PACO40202 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Cell surface A33 antigen protein (22-235AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: GPA33 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 kDa. Observed band size: 50 kDa. |
 | Immunofluorescence staining of MCF-7 cells with PACO40202 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO40202 at dilution of 1:100. |
| バックグラウンド: | May play a role in cell-cell recognition and signaling. |
| シノニム: | Cell surface A33 antigen (Glycoprotein A33), GPA33 |
| UniProt Protein Function: | GPA33: May play a role in cell-cell recognition and signaling. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 1q24.1 Cellular Component: integral to plasma membrane Molecular Function:receptor activity |
| NCBI Summary: | The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99795 |
| NCBI GenInfo Identifier: | 5031561 |
| NCBI Gene ID: | 10223 |
| NCBI Accession: | NP_005805.1 |
| UniProt Secondary Accession: | Q99795,Q5VZP6, |
| UniProt Related Accession: | Q99795 |
| Molecular Weight: | 35,632 Da |
| NCBI Full Name: | cell surface A33 antigen |
| NCBI Synonym Full Names: | glycoprotein A33 |
| NCBI Official Symbol: | GPA33 |
| NCBI Official Synonym Symbols: | A33 |
| NCBI Protein Information: | cell surface A33 antigen |
| UniProt Protein Name: | Cell surface A33 antigen |
| UniProt Synonym Protein Names: | Glycoprotein A33 |
| UniProt Gene Name: | GPA33 |
| UniProt Entry Name: | GPA33_HUMAN |
| Antibodies | ELISA Kits |
| Anti-GPA33 Antibody (CAB15127) | Human ChT1 / VSIG1 ELISA Kit |
| Human Cell surface A33 antigen (GPA33) ELISA Kit |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
