Description
| 抗体名: | FZR1 Antibody (PACO42566) |
| 抗体コード: | PACO42566 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Fizzy-related protein homolog protein (1-493AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: FZR1 antibody at 6µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 56, 55, 45 kDa. Observed band size: 56 kDa. |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO42566 at dilution of 1:100. |
| バックグラウンド: | Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. |
| シノニム: | Fizzy-related protein homolog (Fzr) (CDC20-like protein 1) (Cdh1/Hct1 homolog) (hCDH1), FZR1, CDH1 FYR FZR KIAA1242 |
| UniProt Protein Function: | FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: anaphase-promoting complex; cytosol; nuclear membrane; nucleoplasm Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition DNA damage checkpoint; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle |
| UniProt Code: | Q9UM11 |
| NCBI GenInfo Identifier: | 37537753 |
| NCBI Gene ID: | 51343 |
| NCBI Accession: | Q9UM11.2 |
| UniProt Secondary Accession: | Q9UM11,O75869, Q86U66, Q96NW8, Q9UI96, Q9ULH8, Q9UM10 Q9UNQ1, Q9Y2T8, |
| UniProt Related Accession: | Q9UM11 |
| Molecular Weight: | 44,874 Da |
| NCBI Full Name: | Fizzy-related protein homolog |
| NCBI Synonym Full Names: | fizzy/cell division cycle 20 related 1 |
| NCBI Official Symbol: | FZR1 |
| NCBI Official Synonym Symbols: | FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C |
| NCBI Protein Information: | fizzy-related protein homolog |
| UniProt Protein Name: | Fizzy-related protein homolog |
| UniProt Synonym Protein Names: | CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1 |
| Protein Family: | Fizzy-related protein |
| UniProt Gene Name: | FZR1 |
| UniProt Entry Name: | FZR_HUMAN |
