The ERK1/2 Rabbit Monoclonal Antibody (CAB4782) is a high-quality antibody designed for research involving the extracellular signal-regulated kinases 1 and 2 (ERK1/2). These proteins are key players in the MAPK signaling pathway, involved in various cellular processes such as proliferation, differentiation, and survival.Raised in rabbits, this antibody is highly specific and sensitive for use in Western blotting, immunoprecipitation, and immunofluorescence applications. It binds specifically to ERK1/2 proteins, enabling accurate detection and analysis in various cell types and tissues.
ERK1/2 are known to be dysregulated in many diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. Understanding the role and activity of ERK1/2 is essential for developing targeted therapies and identifying potential drug targets.With its high reactivity and specificity, the ERK1/2 Rabbit Monoclonal Antibody (CAB4782) is an invaluable tool for researchers studying the MAPK signaling pathway and its implications in various diseases.
Product Name:
ERK1/2 Rabbit Monoclonal Antibody
SKU:
CAB4782
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-360 of human ERK2 (P28482).
Sequence:
HTGF LTEY VATR WYRA PEIM LNSK GYTK SIDI WSVG CILA EMLS NRPI FPGK HYLD QLNH ILGI LGSP SQED LNCI INLK ARNY LLSL PHKN KVPW NRLF PNAD SKAL DLLD KMLT FNPH KRIE VEQA LAHP YLEQ YYDP SDEP IAEA PFKF DMEL DDLP KEKL KELI FEET ARFQ PGYR S
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Calculated MW:
42,44kDa
Observed MW:
42kDa/44kDa
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Purification Method:
Affinity purification
Gene ID:
5594/5595
Clone Number:
ARC0212
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ERK1/2 Rabbit mAb (CAB4782) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
