Description
| Product Name: | EOMES Polyclonal Antibody |
| SKU: | CAB18222 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 463-686 of human EOMES (NP_005433.2). |
| Sequence: | QIVP GGRY GVQS FFPE PFVN TLPQ ARYY NGER TVPQ TNGL LSPQ QSEE VANP PQRW LVTP VQQP GTNK LDIS SYES EYTS STLL PYGI KSLP LQTS HALG YYPD PTFP AMAG WGGR GSYQ RKMA AGLP WTSR TSPT VFSE DQLS KEKV KEEI GSSW IETP PSIK SLDS NDSG VYTS ACKR RRLS PSNS SNEN SPSI KCED INAE EYSK DTSK GMGG YYAF YTTP |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | TBR2; EOMES |
| Positive Sample: | Rat brain |
| Cellular Localization: | nucleus |
| Calculated MW: | 73kDa |
| Observed MW: | 70kDa |
This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants.
| Purification Method: | Affinity purification |
| Gene ID: | 8320 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of Rat brain, using EOMES antibody (CAB18222) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
