The EID3 Polyclonal Antibody (PACO19589) is a valuable tool for researchers studying EID3, a protein involved in gene regulation and cell differentiation. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the EID3 protein, this antibody enables the detection and analysis of EID3 in a variety of cell types, making it ideal for studies in cell biology and developmental research.EID3, also known as EP300-interacting inhibitor of differentiation 3, plays a crucial role in the control of gene expression and cell fate determination.
Its involvement in processes such as cell proliferation and differentiation makes it an intriguing target for investigation in the fields of developmental biology and cancer research. Understanding the functions of EID3 can provide valuable insights into pathways that drive disease progression and may lead to the development of targeted therapies in the future.
Antibody Name:
EID3 Antibody (PACO19589)
Antibody SKU:
PACO19589
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human EID3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO19589(EID3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using PACO19589(EID3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
EID3 is highly expressed in testis and shows homology to a region of EID1 implicated in binding to CBP/p300. EID3 acts as a potent inhibitor of nuclear receptor transcriptional activity by a mechanism that is independent of direct interactions with nuclear receptors, including SHP. Furthermore, EID3 directly binds to and blocks the SRC-1 interacting domain of CBP, which has been implicated to act as the interaction surface for nuclear receptor co-activators. Consistent with this idea, EID3 prevents recruitment of CBP to a natural nuclear receptor-regulated promoter.
Synonyms:
EP300 interacting inhibitor of differentiation 3
UniProt Protein Function:
EID3: Tissue-specific component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Belongs to the NSE4 family.Chromosomal Location of Human Ortholog: 12q23.3Cellular Component: chromosome, telomeric region; cytoplasm; nucleusMolecular Function: identical protein binding; protein bindingBiological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA repair; DNA recombination
E1A-like inhibitor of differentiation 3; EID-1-like inhibitor of differentiation 3; Non-structural maintenance of chromosomes element 4 homolog B; NS4EB; Non-SMC element 4 homolog B
