Description
| 抗体名: | DUSP15 Antibody (PACO62207) |
| 抗体コード: | PACO62207 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Dual specificity protein phosphatase 15 protein (1-295AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO62207 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO62207 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| バックグラウンド: | May dephosphorylate MAPK13, ATF2, ERBB3, PDGFRB and SNX6. |
| シノニム: | Dual specificity protein phosphatase 15 (EC 3.1.3.16) (EC 3.1.3.48) (VH1-related member Y) (Vaccinia virus VH1-related dual-specific protein phosphatase Y), DUSP15, C20orf57 VHY |
| UniProt Protein Function: | DUSP15: belongs to the non-receptor class of the protein-tyrosine phosphatase family. The encoded protein has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.48 Chromosomal Location of Human Ortholog: 20q11.21 Molecular Function:protein binding Biological Process: positive regulation of JNK cascade; regulation of cell proliferation; transforming growth factor beta receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. This protein may function in the differentiation of oligodendrocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| UniProt Code: | Q9H1R2 |
| NCBI GenInfo Identifier: | 30316387 |
| NCBI Gene ID: | 128853 |
| NCBI Accession: | Q9H1R2.4 |
| UniProt Secondary Accession: | Q9H1R2,Q5QP62, Q5QP63, Q5QP65, Q6PGN7, Q8N826, Q9BX24 A6NH79, A8MVC8, |
| UniProt Related Accession: | Q9H1R2 |
| Molecular Weight: | 14,721 Da |
| NCBI Full Name: | Dual specificity protein phosphatase 15 |
| NCBI Synonym Full Names: | dual specificity phosphatase 15 |
| NCBI Official Symbol: | DUSP15 |
| NCBI Official Synonym Symbols: | VHY; C20orf57 |
| NCBI Protein Information: | dual specificity protein phosphatase 15 |
| UniProt Protein Name: | Dual specificity protein phosphatase 15 |
| UniProt Synonym Protein Names: | VH1-related member Y |
| Protein Family: | Dual specificity protein phosphatase |
| UniProt Gene Name: | DUSP15 |
| UniProt Entry Name: | DUS15_HUMAN |
