Description
| 抗体名: | DHX58 Antibody (PACO43968) |
| 抗体コード: | PACO43968 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
| 反応性: | Human, Rat |
| 免疫原: | Recombinant Human Probable ATP-dependent RNA helicase DHX58 protein (389-678AA) |
| 憲法: | Liquid |
| ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: DHX58 antibody at 12µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Rat kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 77 kDa. Observed band size: 77 kDa. |
 | Immunohistochemistry of paraffin-embedded human colon cancer using PACO43968 at dilution of 1:100. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using PACO43968 at dilution of 1:100. |
| バックグラウンド: | Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. |
| シノニム: | Probable ATP-dependent RNA helicase DHX58 (EC 3.6.4.13) (Probable ATP-dependent helicase LGP2) (Protein D11Lgp2 homolog) (RIG-I-like receptor 3) (RLR-3) (RIG-I-like receptor LGP2) (RLR), DHX58, D11LGP2E LGP2 |
| UniProt Protein Function: | DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily. |
| UniProt Protein Details: | Protein type:Helicase; Inhibitor; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm Molecular Function:ATP binding; DNA binding; double-stranded RNA binding; helicase activity; protein binding; single-stranded RNA binding; zinc ion binding Biological Process: defense response to virus; innate immune response; metabolic process; negative regulation of innate immune response; negative regulation of interferon type I production; positive regulation of interferon type I production; regulation of innate immune response; response to virus; viral reproduction |
| UniProt Code: | Q96C10 |
| NCBI GenInfo Identifier: | 50401123 |
| NCBI Gene ID: | 79132 |
| NCBI Accession: | Q96C10.1 |
| UniProt Secondary Accession: | Q96C10,Q9HAM6, |
| UniProt Related Accession: | Q96C10 |
| Molecular Weight: | 76,613 Da |
| NCBI Full Name: | Probable ATP-dependent RNA helicase DHX58 |
| NCBI Synonym Full Names: | DEXH-box helicase 58 |
| NCBI Official Symbol: | DHX58 |
| NCBI Official Synonym Symbols: | LGP2; RLR-3; D11LGP2; D11lgp2e |
| NCBI Protein Information: | probable ATP-dependent RNA helicase DHX58 |
| UniProt Protein Name: | Probable ATP-dependent RNA helicase DHX58 |
| UniProt Synonym Protein Names: | Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor LGP2; RLR |
| UniProt Gene Name: | DHX58 |
| UniProt Entry Name: | DHX58_HUMAN |
