Description
| 抗体名: | CYBRD1 Antibody (PACO59708) |
| 抗体コード: | PACO59708 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IF:1:200-1:500 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Cytochrome b reductase 1 protein (220-286AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of Hela cells with PACO59708 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| バックグラウンド: | Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells. |
| シノニム: | Cytochrome b reductase 1 (EC 1) (Duodenal cytochrome b) (Ferric-chelate reductase 3), CYBRD1, DCYTB FRRS3 |
| UniProt Protein Function: | CYBRD1: Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Transporter, ion channel; Transporter; Membrane protein, multi-pass; EC 1.-.-.-; Transporter, iron; Oxidoreductase Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: brush border membrane; integral to membrane; plasma membrane Molecular Function:oxidoreductase activity, oxidizing metal ions; metal ion binding; ferric-chelate reductase activity Biological Process: cellular iron ion homeostasis; response to iron ion; transmembrane transport |
| NCBI Summary: | This gene is a member of the cytochrome b(561) family that encodes an iron-regulated protein. It highly expressed in the duodenal brush border membrane. It has ferric reductase activity and is believed to play a physiological role in dietary iron absorption. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q53TN4 |
| NCBI GenInfo Identifier: | 74726934 |
| NCBI Gene ID: | 79901 |
| NCBI Accession: | Q53TN4.1 |
| UniProt Secondary Accession: | Q53TN4,Q6KC16, Q6KC17, Q6P147, Q6ZR51, Q9H0Q8, Q9H5G5 B2RE79, B4DWD7, |
| UniProt Related Accession: | Q53TN4 |
| Molecular Weight: | 25,087 Da |
| NCBI Full Name: | Cytochrome b reductase 1 |
| NCBI Synonym Full Names: | cytochrome b reductase 1 |
| NCBI Official Symbol: | CYBRD1 |
| NCBI Official Synonym Symbols: | DCYTB; FRRS3; CYB561A2 |
| NCBI Protein Information: | cytochrome b reductase 1; duodenal cytochrome b; ferric-chelate reductase 3; cytochrome b561 family, member A2 |
| UniProt Protein Name: | Cytochrome b reductase 1 |
| UniProt Synonym Protein Names: | Duodenal cytochrome b; Ferric-chelate reductase 3 |
| Protein Family: | Cytochrome b reductase |
| UniProt Gene Name: | CYBRD1 |
| UniProt Entry Name: | CYBR1_HUMAN |
