The CHTOP Antibody (PAC047254) is a polyclonal antibody designed for research involving the CHTOP protein. CHTOP is a critical regulator of mRNA processing and gene expression, playing a key role in various cellular processes such as transcription, splicing, and export of mRNA. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.By specifically binding to the CHTOP protein, this antibody enables researchers to detect and analyze CHTOP expression in different cell types, providing valuable insights for studies in molecular biology and gene regulation.
Understanding the function of CHTOP is essential for unraveling its role in gene expression and its potential implications in disease states like cancer, neurodegenerative disorders, and developmental abnormalities.With its specificity and reliability, the CHTOP Antibody is a valuable tool for advancing research in fields such as genetics, cell biology, and oncology. Researchers can utilize this antibody to uncover the mechanisms underlying CHTOP-mediated gene regulation and explore its therapeutic potential in targeting diseases associated with aberrant mRNA processing.
Western blot. All lanes: CHTOP antibody at 14 µg/ml. Lane 1: Mouse heart tissue. Lane 2: Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 27, 22 kDa. Observed band size: 27, 52 kDa..
Immunohistochemistry of paraffin-embedded human glioma using PACO47254 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO47254 at dilution of 1:100.
Background:
Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis.
Synonyms:
Chromatin target of PRMT1 protein (Friend of PRMT1 protein) (Small arginine- and glycine-rich protein) (SRAG), CHTOP, C1orf77 FOP
UniProt Protein Function:
CHTOP: Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. 3 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-binding; NucleolusChromosomal Location of Human Ortholog: 1q21.3Cellular Component: intracellular membrane-bound organelle; nuclear speck; nucleoplasmMolecular Function: methyl-CpG binding; protein bindingBiological Process: mRNA 3'-end processing; mRNA export from nucleus; positive regulation of ATPase activity; positive regulation of cell proliferation; positive regulation of helicase activity; positive regulation of histone methylation; RNA export from nucleus; termination of RNA polymerase II transcription
UniProt Protein Details:
NCBI Summary:
This gene encodes a small nuclear protein that is characterized by an arginine and glycine rich region. This protein may have an important role in the regulation of fetal globin gene expression and in the activation of estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastomagenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2015]
