Description
| 抗体名: | CDC7 Antibody (PACO44213) |
| 抗体コード: | PACO44213 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Cell division cycle 7-related protein kinase protein (275-574AA) |
| 憲法: | Liquid |
| ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO44213 at dilution of 1:100. |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO44213 at dilution of 1:100. |
| バックグラウンド: | Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. |
| シノニム: | Cell division cycle 7-related protein kinase (CDC7-related kinase) (HsCdc7) (huCdc7) (EC 2.7.11.1), CDC7, CDC7L1 |
| UniProt Protein Function: | CDC7: an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Other group; CDC7 family Chromosomal Location of Human Ortholog: 1p22 Cellular Component: nucleoplasm; microtubule cytoskeleton; intercellular bridge; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; DNA replication initiation; cell division; positive regulation of cell proliferation; mitotic cell cycle; DNA replication; phagocytosis; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle |
| NCBI Summary: | This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008] |
| UniProt Code: | O00311 |
| NCBI GenInfo Identifier: | 12643528 |
| NCBI Gene ID: | 8317 |
| NCBI Accession: | O00311.1 |
| UniProt Secondary Accession: | O00311,O00558, Q5T5U5, D3DT31, |
| UniProt Related Accession: | O00311 |
| Molecular Weight: | 574 |
| NCBI Full Name: | Cell division cycle 7-related protein kinase |
| NCBI Synonym Full Names: | cell division cycle 7 |
| NCBI Official Symbol: | CDC7 |
| NCBI Official Synonym Symbols: | Hsk1; CDC7L1; HsCDC7; huCDC7 |
| NCBI Protein Information: | cell division cycle 7-related protein kinase; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1 |
| UniProt Protein Name: | Cell division cycle 7-related protein kinase |
| Protein Family: | Cell division control protein |
| UniProt Gene Name: | CDC7 |
| UniProt Entry Name: | CDC7_HUMAN |
