Description
| Product Name: | Canine IL 8 Recombinant Protein |
| Product Code: | RPPB1169 |
| Size: | 20µg |
| Species: | Canine |
| Target: | IL 8 |
| Synonyms: | Interleukin-8, IL-8, C-X-C motif chemokine 8, IL8, CXCL8. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 1�PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized�IL-8 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | AVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLFN GNEVCLDPKE KWVQKVVQIF LKKAEKQDP |
| Biological Activity: | The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/ml. |
Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies.When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.
Interleukin-8 Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 79 amino acids and having a molecular mass of 9.1kDa. The IL8 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. |
| UniProt Code: | P41324 |
| NCBI GenInfo Identifier: | 55741690 |
| NCBI Gene ID: | 403850 |
| NCBI Accession: | NP_001003200.1 |
| UniProt Related Accession: | P41324 |
| Molecular Weight: | 11,280 Da |
| NCBI Full Name: | interleukin-8 |
| NCBI Official Symbol: | CXCL8�� |
| NCBI Official Synonym Symbols: | IL8�� |
| NCBI Protein Information: | interleukin-8; IL-8; C-X-C motif chemokine 8; chemokine (C-X-C motif) ligand 8 |
| UniProt Protein Name: | Interleukin-8 |
| UniProt Synonym Protein Names: | C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8 |
| UniProt Gene Name: | CXCL8�� |
| UniProt Entry Name: | IL8_CANFA |
