Description
| Product Name: | Bovine Vimentin Recombinant Protein |
| Product Code: | RPPB3061 |
| Size: | 10µg |
| Species: | Bovine |
| Target: | Vimentin |
| Synonyms: | Vimentin, Vim, FLJ36605. |
| Source: | Bovine Lens |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein (1mg/ml) was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate pH 7.5, 6M urea, 2mM DTT, 1mM EDTA and 10mM methylammonium chloride. |
| Solubility: | It is recommended to reconstitute the Bovine Vimentin in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Bovine Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BovineVimentin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by SDS-PAGE. |
Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.
Bovine Vimentin protein produced in Bovine Lens, having a molecular weight of 57 kDa.
| UniProt Protein Function: | Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Protein type: Motility/polarity/chemotaxis; CytoskeletalCellular Component: neuron projection; focal adhesion; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosolMolecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lensBiological Process: Bergmann glial cell differentiation; in utero embryonic development; intermediate filament organization; astrocyte development |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P48616 |
| NCBI GenInfo Identifier: | 110347570 |
| NCBI Gene ID: | 280955 |
| NCBI Accession: | NP_776394.2 |
| UniProt Secondary Accession: | P48616,Q17QM7 |
| UniProt Related Accession: | P48616 |
| Molecular Weight: | 53,728 Da |
| NCBI Full Name: | vimentin |
| NCBI Synonym Full Names: | vimentin |
| NCBI Official Symbol: | VIM�� |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | vimentin |
| UniProt Protein Name: | Vimentin |
| UniProt Synonym Protein Names: | |
| Protein Family: | Vimentin |
| UniProt Gene Name: | VIM�� |
| UniProt Entry Name: | VIME_BOVIN |
