Description
| Product Name: | Biotinylated Recombinant Human TNFSF5/CD40 Ligand/CD154 Trimer Protein (Primary Amine Labeling) |
| SKU: | RPCB0645 |
| Size: | 100µg |
| Tag: | N-His&Flag |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human TNFSF5/CD40 Ligand/CD154 Trimer Protein (Primary Amine Labeling) , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE；> 95% by HPLC |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 959 |
CD40 ligand or CD40L, also called CD154,? is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P29965 |
 | Biotinylated Human CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | The purity of Biotinylated Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC. |
