Description
| Product Name: | Biotinylated Recombinant Human HMGB1 Protein (Primary Amine Labeling) |
| SKU: | RPCB0546 |
| Size: | 100µg |
| Tag: | C-His |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human HMGB1 Protein (Primary Amine Labeling) , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE；> 95% by HPLC |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 3146 |
High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P09429-1 |
 | Biotinylated Human HMGB1 on Tris- Bis PAGE under reduced condition. The purity is greater than 95%. |
 | The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC. |
