Description
| Product Name: | Biotinylated Recombinant Human Glypican-3/GPC3 Protein |
| SKU: | RPCB0050 |
| Size: | 100µg |
| Tag: | C-His&Avi |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Glypican-3/GPC3 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | > 95% by Tris-Bis PAGE |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 2719 |
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
| Storage: | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P51654 |
 | Biotinylated Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. |
 | Use Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559) , His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3. |
