Description
| Product Name: | Biotinylated Recombinant Human CD3 epsilon Protein |
| SKU: | RPCB0683 |
| Size: | 100µg |
| Tag: | C-His |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human CD3 epsilon Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 916 |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P07766 |
 | Biotinylated Human CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | Immobilized Anti-CD3 Antibody, hFc Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.9ng/mL determined by ELISA. |
