Description
| Product Name: | Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein |
| SKU: | RPCB0047 |
| Size: | 100µg |
| Tag: | N-His&Avi |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | > 95% by Tris-Bis PAGE and HPLC |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation. |
| Gene ID: | 285 |
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
| Storage: | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | O15123 |
 | Biotinylated Human ANGPT2 on Tris- Bis PAGE under reduced condition. The purity is greater than 95%. |
 | Immobilized Biotinylated Human ANGPT2, His Tag at 5μg/ml (100μl/Well) on the Streptavidin Procated plate. Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. |
