Description
| 抗体名: | ATP5H Antibody (PACO45174) |
| 抗体コード: | PACO45174 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | Recombinant Human ATP synthase subunit d, mitochondrial protein (1-161AA) |
| 憲法: | Liquid |
| ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: ATP5H antibody at 5.16 µg/ml. Lane 1: Mouse kidney tissue. Lane 2: Mouse liver tissue. Lane 3: Mouse brain tissue. Lane 4: Hela whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: PC-3 whole cell lysate. Lane 7: U251 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 19, 16 kDa. Observed band size: 19 kDa.. |
 | Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO45174 at dilution of 1:100. |
 | Immunohistochemistry of paraffin-embedded human brain tissue using PACO45174 at dilution of 1:100. |
| バックグラウンド: | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. |
| シノニム: | ATP synthase subunit d, mitochondrial (ATPase subunit d), ATP5H |
| UniProt Protein Function: | ATP5H: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase d subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14 Chromosomal Location of Human Ortholog: 17q25 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex Molecular Function:ATPase activity; transmembrane transporter activity; hydrogen ion transmembrane transporter activity Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport |
| NCBI Summary: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010] |
| UniProt Code: | O75947 |
| NCBI GenInfo Identifier: | 6831494 |
| NCBI Gene ID: | 10476 |
| NCBI Accession: | O75947.3 |
| UniProt Secondary Accession: | O75947,Q9H3J4, B2R5L6, |
| UniProt Related Accession: | O75947 |
| Molecular Weight: | Observed MW 18kDaCalculated MW 15kDa/18kDa |
| NCBI Full Name: | ATP synthase subunit d, mitochondrial |
| NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d |
| NCBI Official Symbol: | ATP5H |
| NCBI Official Synonym Symbols: | ATPQ |
| NCBI Protein Information: | ATP synthase subunit d, mitochondrial; My032 protein; ATPase subunit d; ATP synthase D chain, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d |
| UniProt Protein Name: | ATP synthase subunit d, mitochondrial |
| UniProt Gene Name: | ATP5H |
| UniProt Entry Name: | ATP5H_HUMAN |
