Anti-ARF1 抗体 (PACO51534)

抗体名:	ARF1 Antibody (PACO51534)
抗体コード:	PACO51534
サイズ:	50ug
宿主種:	Rabbit
申し込み:	ELISA, IHC, IF
推奨される希釈:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200
反応性:	Human
免疫原:	Recombinant Human ADP-ribosylation factor 1 protein (2-181AA)

憲法:	Liquid
ストレージバッファ:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
精製方法:	>95%, Protein G purified
抗体のクローン性:	Polyclonal
アイソタイプ:	IgG
Conjugate:	Non-conjugated

	Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO51534 at dilution of 1:100.
	Immunofluorescent analysis of Hela cells using PACO51534 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human lung tissue using PACO51534 at dilution of 1:100.

バックグラウンド:

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

シノニム:

ADP-ribosylation factor 1, ARF1

UniProt Protein Function:	ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:	Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric Chromosomal Location of Human Ortholog: 1q42 Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus
NCBI Summary:	ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P84077
NCBI GenInfo Identifier:	51316985
NCBI Gene ID:	375
NCBI Accession:	P84077.2
UniProt Secondary Accession:	P84077,P10947, P32889,
UniProt Related Accession:	P84077
Molecular Weight:	181
NCBI Full Name:	ADP-ribosylation factor 1
NCBI Synonym Full Names:	ADP-ribosylation factor 1
NCBI Official Symbol:	ARF1
NCBI Protein Information:	ADP-ribosylation factor 1
UniProt Protein Name:	ADP-ribosylation factor 1
Protein Family:	Auxin response factor
UniProt Gene Name:	ARF1
UniProt Entry Name:	ARF1_HUMAN

Antibodies

Anti-ARF1 Antibody (CAB16345)

Anti-ARF1 Antibody (CAB2821)

Anti-ARF1 Antibody (CAB7643)

Anti-ARF1 Antibody (CAB19977)[KO Validated]

Anti-ARF1 Antibody (CAB9195)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)