The APPL1 Polyclonal Antibody (CAB4606) is a valuable tool for researchers studying APPL1, a key adaptor protein involved in various cellular processes. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By targeting the APPL1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in cell signaling, metabolism, and cancer research.APPL1, also known as adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1, plays a crucial role in regulating insulin signaling, vesicle trafficking, and cell survival.
Its involvement in multiple signaling pathways makes it a promising candidate for research into metabolic disorders, cancer progression, and neurodegenerative diseases. Investigating the function of APPL1 is essential for gaining insights into these complex diseases and developing targeted therapies to address them.
Product Name:
APPL1 Rabbit Polyclonal Antibody
SKU:
CAB4606
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human APPL1 (NP_036228.1).
Early endosome membrane, Nucleus, Peripheral membrane protein.
Calculated MW:
80kDa
Observed MW:
95kDa
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
Purification Method:
Affinity purification
Gene ID:
26060
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using APPL1 antibody (CAB4606) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
