The RAD21 Polyclonal Antibody (CAB18850) is a valuable tool for researchers studying RAD21, a key protein involved in chromosome condensation and DNA repair processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the RAD21 protein, this antibody allows for the detection and analysis of RAD21 levels in various cell types, making it ideal for studies in genetics, cell biology, and cancer research.
RAD21 plays a critical role in maintaining genomic stability and proper chromosome segregation during cell division. Dysregulation of RAD21 has been linked to various genetic disorders and cancer development, highlighting the importance of studying its function in disease mechanisms. By utilizing the RAD21 Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms involving RAD21 and its impact on cellular processes.
Product Name:
Rad21 Rabbit Polyclonal Antibody
SKU:
CAB18850
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 281-420 of human Rad21 (NP_006256.1).
condensed nuclear chromosome, cytosol, nuclear matrix, nucleoplasm, nucleus, spindle pole
Calculated MW:
72kDa
Observed MW:
130kDa
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Purification Method:
Affinity purification
Gene ID:
5885
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Rad21 Rabbit pAb (CAB18850) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
