The Anti-Phospho-NMDAR1 (S896/S897) Antibody (CABP0962) is a valuable tool for researchers studying NMDAR1, a key receptor in excitatory neurotransmission in the brain. This antibody, produced in rabbits, is highly specific for phosphorylated NMDAR1 at serine 896 and 897 residues, allowing for precise detection and analysis in various experimental settings. It is validated for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation.Phosphorylation of NMDAR1 at serine 896 and 897 plays a crucial role in regulating the activity of NMDA receptors, which are involved in synaptic plasticity, learning, and memory. Dysregulation of NMDAR1 phosphorylation has been implicated in neurological disorders such as Alzheimer's disease, schizophrenia, and epilepsy.
By studying the phosphorylation status of NMDAR1, researchers can gain insights into the molecular mechanisms underlying these conditions and identify potential therapeutic targets.Overall, the Anti-Phospho-NMDAR1 (S896/S897) Antibody (CABP0962) is a valuable tool for exploring the role of NMDAR1 phosphorylation in neurological function and dysfunction. Its specificity and reliability make it an ideal choice for researchers interested in unraveling the complex signaling pathways that govern synaptic transmission and synaptic plasticity in the brain.
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
2902
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-NMDAR1-S896/S897 Rabbit pAb (CABP0962) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
