The NUDT5 Polyclonal Antibody (CAB0609) is a valuable tool for researchers investigating NUDT5, an enzyme involved in nucleotide metabolism and cellular signaling pathways. This antibody, generated in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By targeting the NUDT5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in biochemistry and cancer research.
NUDT5, also known as nudix hydrolase 5, plays a crucial role in maintaining cellular health by regulating the levels of nucleotide pools and signaling molecules. Dysregulation of NUDT5 activity has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. Therefore, studying the function of NUDT5 is essential for unraveling its role in disease pathogenesis and identifying potential therapeutic targets for intervention.
Product Name:
NUDT5 Rabbit Monoclonal Antibody
SKU:
CAB0609
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUDT5 (Q9UKK9).
This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.
Purification Method:
Affinity purification
Gene ID:
11164
Clone Number:
ARC2525
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NUDT5 antibody (CAB0609) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
