The Anti-Nesprin-1 Antibody (CAB20256) is a key tool for researchers studying Nesprin-1, a protein involved in cellular structure and function. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to Nesprin-1, this antibody enables precise detection and analysis in a range of cell types, making it an invaluable resource for studies in cell biology and molecular biology.Nesprin-1 is a critical component of the linker of nucleoskeleton and cytoskeleton (LINC) complex, which plays a crucial role in maintaining nuclear structure, gene regulation, and nuclear positioning within the cell.
Dysregulation of Nesprin-1 has been linked to various diseases, including muscular dystrophies, cardiomyopathies, and cancer. Investigations into the function and regulation of Nesprin-1 are essential for understanding its role in these pathologies and may provide insight into potential therapeutic interventions targeting this protein.Researchers interested in cellular mechanics, nuclear organization, and disease mechanisms will find the Anti-Nesprin-1 Antibody (CAB20256) to be a valuable asset in their experimental toolbox. Its specificity and reliability make it a trusted reagent for exploring the intricate roles of Nesprin-1 in health and disease.
Product Name:
Nesprin 1 Rabbit Polyclonal Antibody
SKU:
CAB20256
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 8220-8410 of human Nesprin 1 (NP_892006.3).
This gene encodes a spectrin repeat containing protein expressed in skeletal and smooth muscle, and peripheral blood lymphocytes, that localizes to the nuclear membrane. Mutations in this gene have been associated with autosomal recessive spinocerebellar ataxia 8, also referred to as autosomal recessive cerebellar ataxia type 1 or recessive ataxia of Beauce. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
23345
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse skeletal muscle, using Nesprin 1 Rabbit pAb (CAB20256) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
