Description
| Product Name: | Anti-Mouse NKG2A/C/E In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0384 |
| Size: | 1 mg, 5 mg, 25 mg, 50 mg, 100 mg |
| Clone: | 20D5 |
| Protein: | NKG2a |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD159a, CD159c, CD159e, NKG2C, NKG2E |
| Isotype: | IgG2a k |
| Reactivity: | Mouse |
| Applications: | B, FC, IHC, In Vivo |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Endotoxin Level: | ≤ 0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC ⋅ >95% by SDS Page |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Applications: | B, FC, IHC, In Vivo |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 20D5 recognizes an epitope on the mouse NKG2A, NKG2C, and NKG2E isoforms of the CD94/NKG2 heterodimer. DBA/2J mice are CD94-deficient and do not express CD94/NKG2 receptors. |
| Antigen Distribution: | NKG2A/C/E is expressed on NK cells, NKT cells, and activated CD8 T cells. |
| Immunogen: | CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | ≤ 0.5 EU/mg as determined by the LAL method |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Purity: | ≥98% monomer by analytical SEC ⋅ >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Protein: | NKG2a |
| Function: | Inhibitory activity via ITIMs |
| Research Area: | Cell Biology, Immunology, Signal Transduction, Stem Cell |
| Pathogen Testing: | To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. |
| Shipping: | 2-8°C |
| Molecular Weight: | 150 kDa |
