Anti-Mouse/Human KLRG-1 - Purified (IVMB0401)

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SKU:
IVMB0401
Antibody Type:
Biosimilar Reference Antibody
Applications:
IHC
Applications:
FC
Applications:
Drug Development
Applications:
Pharmacokinetics
Applications:
ICC
Applications:
IP
Applications:
WB
Disease Area:
Cancer
Clone:
2F1
Isotype:
Syrian Hamster IgG
Host Species:
Syrian Hamster
Reactivity:
Human/Mouse
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Description

system_update_altDatasheet
Product Name: Anti-Mouse/Human KLRG-1 – Purified
Product Code: IVMB0401
Size: 10 mg, 25 mg
Clone: 2F1
Target: KLRG-1
Host Species: Syrian Hamster
Product Type: Hybridoma Monoclonal Antibody
Synonyms: KLRG1, 2F1, MAFA
Isotype: Syrian Hamster IgG
Reactivity: Human/Mouse
Applications: FC, ICC, IP, WB
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Concentration: 0.5 mg/ml
State of Matter: Liquid

Killer cell lectin-like receptor G1 (KLRG1) is a transmembrane glycoprotein with an extracellular C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM)1. KLRG1 plays an inhibitory role in human NK and T cells2 and is frequently used as a marker of cellular differentiation, with memory cells and highly differentiated end-stage cells having a significantly higher percentage of expression than differentiating cells2. Additionally, the proportion of CD8+ T cells expressing KLRG1 is age-related, increasing from ~ 40% of the CD8+ T cell pool in young adults to 90% in individuals over 65. KLRG1 is known to bind E-, N-, and R-cadherin1, 2. Cadherin interactions are generally involved in cell-to-cell adhesion and additionally, KLRG1/E-cadherin interactions are involved in immune control2.

2F1 was generated by immunizing Syrian hamsters with IL-2 activated NK (A-LAK) cells from B6 (H-2b) mice3. Popliteal lymph node cells were fused to murine myeloma P3X63Ag.8.653 cells and hybridomas screened for antibodies specific to A-LAK cells.

Antibody 2F1 was used to show that KLRG1 is indirectly upregulated by expression of MHC class I molecules in an interaction affected by class I-specific Ly49 inhibitory receptors and was also used to show that KLRG1 expression is modulated by SHP-1 3 . Additionally, 2F1 was used to identify the KLRG1 mouse homolog and show that it is not expressed on mouse mast cells 4.

Specificity: 2F1 activity is directed against mouse and human KLRG1.
Antigen Distribution: KLRG1 is expressed by subsets of natural killer cells and a small fraction of T cells but not by other lymphohematopoietic cells in both mouse and human.
Immunogen: IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice
Ligand/Receptor: E-, N-, R- cadherins
Storage and Handling: This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Uniprot: Q96E93
NCBI Gene Bank ID: 10219
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