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Description
Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a precision-engineered monoclonal antibody tailored for in vivo research applications. This antibody targets the CD44 protein, a key cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration, making it an invaluable tool for research in immunology, oncology, and stem cell biology. With a rat IgG2b kappa isotype, it ensures ultra-low endotoxin levels (<0.001 EU/μg), optimizing it for sensitive assays such as ELISA, flow cytometry, immunohistochemistry, and beyond. Offered in a variety of sizes, it is formulated in phosphate-buffered saline to guarantee stability and efficacy.
Elevate your research capabilities with this reliable and versatile antibody. CD44 is a glycoprotein ubiquitously expressed on the surface of various cell types, including hematopoietic, epithelial, and endothelial cells. It plays a pivotal role in regulating cell migration, proliferation, and survival through its interactions with hyaluronic acid and other ligands. This antibody’s specificity to CD44 enables precise targeting and identification of cells involved in critical biological processes, crucial for developing therapeutic strategies and advancing scientific knowledge.
| Product Name: | Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0079 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | IM7 |
| Protein: | CD44 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Pgp-1, Ly-24, H-CAM |
| Isotype: | Rat IgG2b |
| Reactivity: | Baboon, Chimpanzee, Rhesus, Bovine, Horse, Human, Mouse, Pig |
| Immunogen: | Dexamethasone-induced myeloid leukemia M1 cells |
| Applications: | CyTOF®, ELISA Det, FC, ICC, IHC FF, IHC FFPE, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, CyTOF®, ELISA Det, FC, ICC, IHC FF, IHC FFPE, IP, WB |
| Recommended Usage: | FC The suggested concentration for this IM7 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this P84 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Baboon, Chimpanzee, Rhesus, Bovine, Horse, Human, Mouse, Pig |
| Host Species: | Rat |
| Specificity: | Clone IM7 recognizes an epitope common to alloantigens and all isoforms of CD44 that is located between amino acids 145 and 186. |
| Antigen Distribution: | CD44 is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells in addition to B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells. |
| Immunogen: | Dexamethasone-induced myeloid leukemia M1 cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD44 is an 80-95 kD glycoprotein that plays a role in various cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. CD44 interacts with osteopontin, collagens, and matrix metalloproteinases (MMPs) and is a receptor for hyaluronic acid. Transcripts for this gene go through intricate alternative splicing that result in a variety of functionally distinct isoforms, including those which may be related to tumor metastasis. These splice variants of CD44 function as receptors under hemodynamic flow conditions that are significant to the development of cancer metastasis. Hence, it is thought that anti-CD44 tumor-specific mAbs may have therapeutic potential. This therapeutic potential of anti-CD44 mAbs is evident in some animal experiments demonstrating a reduction in malignant activities of various neoplasms when CD44 was targeted by a combination of mAbs, antisense oligonucleotides, and CD44-soluble proteins. It has been reported that high levels of CD44 on leukemic cells fuel leukemia production. Notably, various cancer studies show conflicting results pertaining to level of CD44 expression and its correlation with disease prognosis. Before anti-CD44 therapy can be applied to human cancers, it is essential to resolve this inconsistency.
| Technical Datasheet: | View |
| Protein: | CD44 |
| Ligand/Receptor: | Hyaluronan, MIP-1β, fibronectin, collagen |
| Research Area: | Cell Adhesion, Cell Biology, Immunology, Stem Cell |
| Rat IgG2b Isotype Control | |
|---|---|
| Clone | 1-2 |
| Isotype | Rat IgG2b kappa |
| Endotoxin Level | Ultra Low Endotoxin |
![Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Low Endotoxin (IVMB0078)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/590x590/products/172989/172760/anti-mousehuman-cd44-im7-in-vivo-antibody-low-endotoxin__61349__80219.1750448976.jpg?c=1 "Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Low Endotoxin (IVMB0078)")
