Anti-Mouse CD3epsilon [500A2] In Vivo Antibody - Ultra Low Endotoxin (IVMB0151)

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SKU:
IVMB0151
Antibody Type:
Functional-Grade In Vivo Antibody
Applications:
In Vivo
Disease Area:
Cancer
Clone:
500A2
Protein:
CD3-epsilon
Isotype:
Syrian Hamster IgG2a kappa
Reactivity:
Mouse
Synonyms:
T3
Synonyms:
CD3
Research Area:
T Cell Depletion
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Syrian Hamster
Applications:
Blocking
Applications:
FC
Applications:
IHC FF
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Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse CD3ε [500A2] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0151
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:500A2
Protein:CD3-epsilon
Product Type:Monoclonal Antibody
Synonyms:T3, CD3
Isotype:Syrian Hamster IgG2a κ
Reactivity:Mouse
Immunogen:Mouse T-cell receptor
Applications:B, FC, IHC FF
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:≤ 0.5 EU/mg as determined by the LAL method
Purity: ≥98% monomer by analytical SEC
>95% by SDS Page
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0150Anti-Mouse CD3-epsilon [500A2] In Vivo Antibody - Low EndotoxinLow
IVMB0151Anti-Mouse CD3ε [500A2] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, FC, IHC FF
Recommended Usage: FC
The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Syrian Hamster
Specificity:Clone 500A2 recognizes an epitope on mouse CD3-epsilon.
Antigen Distribution: CD3-epsilon is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen:Mouse T-cell receptor
Concentration:≥ 5.0 mg/ml
Endotoxin Level:≤ 0.5 EU/mg as determined by the LAL method
Purity: ≥98% monomer by analytical SEC
>95% by SDS Page
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Technical Datasheet:View
Protein:CD3-epsilon
Ligand/Receptor:Peptide antigen/MHC-complex.
Research Area:Cell Biology, Immunology, Signal Transduction
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