Anti-Mouse CD226 (DNAM-1) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD226 (DNAM-1) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody crafted for in vivo applications. This antibody targets the CD226 (DNAM-1) protein, a vital molecule in the modulation of immune responses, making it indispensable for research in immunology and related fields. With a rat IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), ideal for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline to ensure stability and efficacy.
Enhance your research with this robust and versatile antibody. CD226 (DNAM-1) is a glycoprotein expressed on the surface of immune cells such as natural killer (NK) cells and some T cells. It plays a considerable role in the immune system, particularly in the interactions between NK cells and target cells, and in the co-stimulation of T cells. This makes Anti-Mouse CD226 (DNAM-1) Antibody an excellent tool for investigations into immune cell regulation, cellular cytotoxicity, and disease pathogenesis.
Product Name:
Anti-Mouse CD226 (DNAM-1) In Vivo Antibody - Low Endotoxin
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FC, In Vivo
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone 480.1 activity is directed against murine CD226 (DNAM-1; PTA-1).
Antigen Distribution:
mCD226 is expressed on the surface of differentiated Th1 cells, CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and activated CD4+ cells.
Immunogen:
Th1 polarized T cell clones
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signalling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and NK cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK-mediated killing of immature dendritic cells2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3.
CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding.
mCD226 is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. In vivo treatment with anti-CD226 (clone 10E5) inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease. Additionally, mCD226 is constitutively expressed on CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and is up-regulated on activated CD4+ cells2,3. mCD226 is a marker for mature T cells2.
