Anti-Human CD45 [T29/33] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD45 [T29/33] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody developed for in vivo applications. This antibody targets the CD45 protein, a critical regulator of immune cell signaling, making it ideal for research in immunology and related fields. With a mouse IgG1 kappa isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), suitable for a range of assays including ELISA, flow cytometry, and immunohistochemistry. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD45, also known as protein tyrosine phosphatase receptor type C (PTPRC), is expressed on the surface of all nucleated hematopoietic cells. It plays a pivotal role in the immune system by regulating the signaling threshold of lymphocyte activation and differentiation, significantly impacting the development and function of T-cells, B-cells, and other immunity-related cells.
Product Name:
Anti-Human CD45 (Clone T29/33) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0310
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
T29/33
Protein:
CD45
Product Type:
Monoclonal Antibody
Synonyms:
LCA [Leukocyte Common Antigen]
Isotype:
IgG2b
Reactivity:
Human
Applications:
FC, In Vivo
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FC, In Vivo
Reactivity:
Human
Host Species:
Mouse
Specificity:
Clone T29/33 recognizes an epitope on human CD45.
Antigen Distribution:
The CD45 antigen is present on all human leukocytes and progenitors in bone marrow. This antibody binds to all human hematopoietic cells, but not to non-hematopoietic cells. Anti-CD45 (T29/33) is not cytotoxic in the presence of complement.
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A,B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.
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