Description
Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD11c protein, crucial in immune response, making it ideal for research in immunology and related fields.
With a mouse IgG1 isotype, it ensures ultra-low endotoxin levels (<0.002 EU/µg), providing exceptional purity and performance for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. CD11c is an integrin protein predominantly found on the surface of dendritic cells, monocytes, macrophages, and some subsets of T cells. It plays a significant role in the immune system by mediating cellular adhesion and signaling processes, contributing to the activation and function of immune cells. This makes the Anti-Human CD11c In Vivo Antibody an essential tool for the study of immune cell dynamics and functions in various disease models.
| Product Name: | Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0110 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 3.9 |
| Protein: | CD11c |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Integrin αX subunit, CR4, p150, ITGAX |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Human |
| Immunogen: | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
| Applications: | B, CyTOF®, FA, FC, IHC FF, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FA, FC, IHC FF, WB |
| Recommended Usage: | FC The suggested concentration for clone 3.9 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.> WB The suggested concentration for this 3.9 antibody for use in western blotting is 1-10 µg/ml. A suggested positive control for Western blotting is a Mouse Skeletal Muscle Tissue Lysate. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone 3.9 recognizes the α-chain (CD11c) of the CD11c/CD18 complex. It is specific for the I domain of CD11c. Clone 3.9 binds the activated form of CD11c and partially blocks the binding of CD11c with ICAM-4. |
| Antigen Distribution: | CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. |
| Immunogen: | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11c |
| Function: | Adhesion, CTL killing |
| Ligand/Receptor: | CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
