Description
Anti-Human CD11a In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human CD11a In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD11a protein, a critical component in leukocyte adhesion and signaling, making it indispensable for research in immunology, cell biology, and related fields. With a mouse IgG2b kappa isotype, this antibody ensures high purity and ultra-low endotoxin levels (<0.01 EU/μg), suitable for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline to ensure stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD11a, also known as Leukocyte Function-associated Antigen 1 (LFA-1), is a part of the integrin family and expressed on the surface of leukocytes. It plays a significant role in the immune system by mediating the adhesion and migration of leukocytes to sites of inflammation and facilitating the immune response through interaction with intercellular adhesion molecules (ICAMs).
| Product Name: | Anti-Human CD11a In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0106 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 38 |
| Protein: | CD11a |
| Product Type: | Monoclonal Antibody |
| Synonyms: | LFA-1α chain, ITGAL |
| Isotype: | Mouse IgG2a |
| Reactivity: | Human |
| Immunogen: | Fibronectin purified monocytes. |
| Applications: | Costim, FC, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | Costim, FC, WB |
| Recommended Usage: | FC The suggested concentration for this 38 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB This antibody can be used to detect Human, Mouse and Rat SHP2 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, under either reducing or non-reducing conditions. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone 38 recognizes an epitope on human CD11a. |
| Antigen Distribution: | CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. |
| Immunogen: | Fibronectin purified monocytes. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11a |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a kappa |
| Endotoxin Level | Ultra Low Endotoxin |
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