The Anti-Complement C4A Antibody (CAB3545) is a highly specific polyclonal antibody designed for research involving Complement C4A, a key component of the complement system involved in immune response regulation. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.Complement C4A is a critical player in the immune system, aiding in the recognition and clearance of pathogens and damaged cells.
Dysregulation of Complement C4A has been implicated in various diseases, making it a valuable target for research in fields such as immunology, infectious diseases, and autoimmune disorders. By targeting Complement C4A with this antibody, researchers can gain insights into the role of this protein in health and disease, paving the way for the development of novel therapeutic strategies.
Product Name:
C4D Rabbit Monoclonal Antibody
SKU:
CAB3545
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 940-1237 of human Complement C4A (P0C0L4).
This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
720
Clone Number:
ARC2037
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Complement C4A Rabbit mAb (CAB3545) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
