The COA Synthase (COASY) Monoclonal Antibody (CAB0928) is a vital tool for researchers investigating the role of COASY in metabolic pathways and lipid metabolism. This antibody, generated in mice, is highly specific and reacts with human samples, making it ideal for use in immunohistochemistry and Western blotting applications. By targeting the COASY protein, this antibody allows for the detection and analysis of COASY in various cell types, offering valuable insights into its function in cellular processes.COASY, also known as coenzyme A synthase, is a key enzyme involved in the biosynthesis of coenzyme A, an essential cofactor in numerous metabolic pathways.
Dysregulation of COASY has been implicated in various metabolic disorders, making it an important target for research in metabolic diseases and lipid-related conditions. Understanding the activity of COASY is crucial for developing treatments that target metabolic pathways and may have implications for diseases such as obesity, diabetes, and neurodegenerative disorders.
Product Name:
COASY Rabbit Monoclonal Antibody
SKU:
CAB0928
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 465-564 of human CoA synthase (COASY） (Q13057).
NBP; DPCK; PPAT; UKR1; NBIA6; PCH12; pOV-2; CoA synthase (COASY）
Positive Sample:
HT-29,293T
Conjugate:
Unmodified
Cellular Localization:
Cytoplasm, Mitochondrion matrix.
Calculated MW:
62kDa
Observed MW:
62kDa
Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms.
Purification Method:
Affinity purification
Gene ID:
80347
Clone Number:
ARC2552
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CoA synthase (COASY） antibody (CAB0928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
