The Anti-Acetyl Histone H4 (K5) Antibody (CAB20397) offered by Assay Genie is a valuable tool for researchers studying epigenetics and chromatin regulation. This polyclonal antibody, produced in rabbits, specifically targets the acetylated histone H4 lysine 5 modification, enabling researchers to investigate the role of this post-translational modification in gene regulation and chromatin remodeling.Histone modifications, such as acetylation, play a crucial role in regulating gene expression and chromatin structure. The Anti-Acetyl Histone H4 (K5) Antibody is highly reactive with human samples and has been validated for use in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation.
Its specificity for the acetylated histone H4 lysine 5 mark enables precise detection and analysis of this modification in various cell types and experimental conditions.By studying the acetylation status of histone H4 lysine 5, researchers can gain insights into the epigenetic mechanisms that control gene expression and cellular function. This antibody is a valuable tool for investigations into gene regulation, chromatin dynamics, and the impact of histone modifications on biological processes. Researchers in fields such as cancer biology, developmental biology, and epigenetics will find the Anti-Acetyl Histone H4 (K5) Antibody essential for their studies.
Product Name:
Acetyl-Histone H4-K5 Rabbit Polyclonal Antibody
SKU:
CAB20397
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
Sequence:
GRGK G
Tested Applications:
WBChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 ChIP,5μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H4-K5 Rabbit pAb (CAB20397) at 1:1000 dilution.HeLa cells,NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
