The AMN Antibody (PAC059445) is a highly specific and sensitive tool for researchers studying the AMN protein, known for its role in vitamin B12 absorption and transport. This polyclonal antibody, generated in rabbits, is optimized for detecting AMN in human samples using Western blot techniques. By binding to the AMN protein, researchers can accurately measure its expression levels across various cell types, aiding in the investigation of vitamin B12-related disorders and potential therapeutic targets.
AMN is a key player in the complex process of vitamin B12 uptake and utilization within the body, making it essential for maintaining proper neurological function and overall health. Dysregulation of AMN function has been linked to conditions such as pernicious anemia, neurological disorders, and developmental abnormalities. By understanding the mechanisms behind AMN activity, researchers can potentially develop targeted interventions to address these health issues and improve patient outcomes.
IHC image of PACO59445 diluted at 1:600 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO59445 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Necessary for efficient absorption of vitamin B12. Required for normal CUBN-mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (By similarity).
Synonyms:
Protein amnionless, AMN
UniProt Protein Function:
Necessary for efficient absorption of vitamin B12 (PubMed:12590260, PubMed:14576052). Required for normal CUBN-mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm ().
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008]
