Description
| 抗体名: | AGPS Antibody (PACO57656) |
| 抗体コード: | PACO57656 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Alkyldihydroxyacetonephosphate synthase, peroxisomal protein (279-400AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate. All lanes: AGPS antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73 kDa. Observed band size: 73 kDa. |
 | Immunofluorescence staining of NIH/3T3 cells with PACO57656 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57656 diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids. |
| シノニム: | Alkyldihydroxyacetonephosphate synthase, peroxisomal (Alkyl-DHAP synthase) (EC 2.5.1.26) (Aging-associated gene 5 protein) (Alkylglycerone-phosphate synthase), AGPS |
| UniProt Protein Function: | AGPS: Defects in AGPS are the cause of rhizomelic chondrodysplasia punctata type 3 (RCDP3). RCDP3 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. Belongs to the FAD-binding oxidoreductase/transferase type 4 family. |
| UniProt Protein Details: | Protein type:EC 2.5.1.26; Lipid Metabolism - ether lipid; Mitochondrial; Oxidoreductase; Transferase Chromosomal Location of Human Ortholog: 2q31.2 Cellular Component: membrane; mitochondrion; nucleolus; peroxisomal matrix; peroxisomal membrane; peroxisome Molecular Function:alkylglycerone-phosphate synthase activity; protein binding Biological Process: ether lipid biosynthetic process; lipid biosynthetic process Disease: Rhizomelic Chondrodysplasia Punctata, Type 3 |
| NCBI Summary: | This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00116 |
| NCBI GenInfo Identifier: | 2498106 |
| NCBI Gene ID: | 8540 |
| NCBI Accession: | O00116.1 |
| UniProt Secondary Accession: | O00116,Q2TU35, A5D8U9, |
| UniProt Related Accession: | O00116 |
| Molecular Weight: | 73kDa |
| NCBI Full Name: | Alkyldihydroxyacetonephosphate synthase, peroxisomal |
| NCBI Synonym Full Names: | alkylglycerone phosphate synthase |
| NCBI Official Symbol: | AGPS |
| NCBI Official Synonym Symbols: | ADAS; ADPS; RCDP3; ADAP-S; ADHAPS; ALDHPSY |
| NCBI Protein Information: | alkyldihydroxyacetonephosphate synthase, peroxisomal |
| UniProt Protein Name: | Alkyldihydroxyacetonephosphate synthase, peroxisomal |
| UniProt Synonym Protein Names: | Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase |
| Protein Family: | Alkyldihydroxyacetonephosphate synthase |
| UniProt Gene Name: | AGPS |
| Antibodies |
| Anti-AGPS Antibody (CAB10484) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
