Description
| 抗体名: | ADH1A Antibody (PACO55382) |
| 抗体コード: | PACO55382 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Alcohol dehydrogenase 1A protein (104-253AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO55382 diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO55382 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO55382 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | cytosol, nucleoplasm, plasma membrane, alcohol dehydrogenase (NAD) activity, alcohol dehydrogenase activity, zinc-dependent, retinol dehydrogenase activity, alcohol metabolic process, drug metabolic process, ethanol oxidation |
| シノニム: | Alcohol dehydrogenase 1A (EC 1.1.1.1) (Alcohol dehydrogenase subunit alpha), ADH1A, ADH1 |
| UniProt Code: | P07327 |
| NCBI GenInfo Identifier: | 4501929 |
| NCBI Gene ID: | 124 |
| NCBI Accession: | NP_000658.1 |
| UniProt Secondary Accession: | P07327,Q17R68, A8K3E3, |
| UniProt Related Accession: | P07327 |
| Molecular Weight: | 375 |
| NCBI Full Name: | alcohol dehydrogenase 1A |
| NCBI Synonym Full Names: | alcohol dehydrogenase 1A (class I), alpha polypeptide |
| NCBI Official Symbol: | ADH1A |
| NCBI Official Synonym Symbols: | ADH1 |
| NCBI Protein Information: | alcohol dehydrogenase 1A; ADH, alpha subunit; aldehyde reductase; alcohol dehydrogenase subunit alpha; alcohol dehydrogenase 1 (class I), alpha polypeptide |
| UniProt Protein Name: | Alcohol dehydrogenase 1A |
| UniProt Synonym Protein Names: | Alcohol dehydrogenase subunit alpha |
| Protein Family: | Alcohol dehydrogenase |
| UniProt Gene Name: | ADH1A |
| UniProt Entry Name: | ADH1A_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
