S1PR1 Antibody (PACO29732)
- SKU:
- PACO29732
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | S1PR1 Antibody (PACO29732) |
抗体コード: | PACO29732 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Sphingosine 1-phosphate receptor 1 protein (311-382AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: S1PR1 antibody at 2µg/ml Lane 1: Jurkat whole cell lysate Lane 2: A375 whole cell lysate Lane 3: Mouse thymus tissue Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa . | |
Immunohistochemistry of paraffin-embedded human breast cancer using PACO29732 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO29732 at dilution of 1:100. |
バックグラウンド: | G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury. |
シノニム: | Sphingosine 1-phosphate receptor 1 (S1P receptor 1) (S1P1) (Endothelial differentiation G-protein coupled receptor 1) (Sphingosine 1-phosphate receptor Edg-1) (S1P receptor Edg-1) (CD antigen CD363), S1PR1, CHEDG1 EDG1 |
UniProt Protein Function: | EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins. |
UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 1p21.2 Cellular Component: intracellular membrane-bound organelle; intrinsic to plasma membrane; nucleoplasm; plasma membrane Molecular Function:G-protein coupled receptor activity; G-protein-coupled receptor binding Biological Process: actin cytoskeleton reorganization; blood vessel maturation; cell adhesion; cell migration; chemotaxis; G-protein coupled receptor protein signaling pathway; lamellipodium biogenesis; regulation of bone mineralization; regulation of bone resorption |
NCBI Summary: | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
UniProt Code: | P21453 |
NCBI GenInfo Identifier: | 205371820 |
NCBI Gene ID: | 1901 |
NCBI Accession: | P21453.2 |
UniProt Secondary Accession: | P21453,Q9BYY4, Q9NYN8, D3DT66, |
UniProt Related Accession: | P21453 |
Molecular Weight: | |
NCBI Full Name: | Sphingosine 1-phosphate receptor 1 |
NCBI Synonym Full Names: | sphingosine-1-phosphate receptor 1 |
NCBI Official Symbol: | S1PR1 |
NCBI Official Synonym Symbols: | EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362 |
NCBI Protein Information: | sphingosine 1-phosphate receptor 1 |
UniProt Protein Name: | Sphingosine 1-phosphate receptor 1 |
UniProt Synonym Protein Names: | Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD_antigen: CD363 |
Protein Family: | Sphingosine 1-phosphate receptor |
UniProt Gene Name: | S1PR1 |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |